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TOR1 AIP1 与p53相互作用,增强前列腺癌进展中的细胞周期失调

Zhaofeng Li¹, Xueyu Li¹, Han Yang¹

¹Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Molecular and cellular biochemistry

|April 8, 2025

概括

编码LAP1蛋白的Tor1aip1基因在前列腺癌 (PRAD) 中被下调. 它稳定p53,通过阻止细胞循环来抑制瘤生长和侵袭,使其成为潜在的PRAD治疗点.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

细胞循环是一个关键的调节器,也是癌症治疗的目标.
由Tor1aip1编码的LAP1蛋白对核外结构和细胞周期进展至关重要.

研究的目的:

调查Tor1aip1基因在前列腺腺癌 (PRAD) 发展中的作用和机制.
评估TOR1AIP1作为PRAD的潜在诊断和治疗点.

主要方法:

从TCGA数据库中分析PRAD患者的TOR1AIP1表达和生存数据.
在前列腺癌组织中验证TOR1AIP1表达,使用qPCR,西斑和免疫组织化学.
在前列腺癌细胞中的功能研究涉及TOR1AIP1过度表达或使用lentiviral载体敲除,通过CCK-8,殖民地形成,Transwell测定,西部斑块和流动细胞计量来评估增殖,入侵和细胞周期.

主要成果:

发现TOR1AIP1表达在PRAD中较低,与瘤阶段和预后相关.
TOR1AIP1直接与p53相互作用,增强其蛋白质稳定性.
提升TOR1AIP1的调控通过诱导S相细胞循环停止来抑制前列腺癌细胞的增殖和侵入.

结论:

在PRAD中,TOR1AIP1通过稳定p53并诱导细胞循环停止,起到瘤抑制作用.
对于PRAD来说,TOR1AIP1是一个有前途的治疗标,有可能用于组合疗法.

关键词:

细胞循环失调的调节障碍这是入侵的入侵.在P53中,P53是P53 核武器的扩散前列腺腺癌前列腺腺癌.在TOR1 AIP1 AIP1 AIP1

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