Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

11.6K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
11.6K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

14.6K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
14.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inverse design of guanine-defects in carbon nanotubes for high-resolution emission tuning.

Science advances·2026
Same author

Arginine attenuates microbiologically influenced corrosion of Ti in Candida albicans-Streptococcus oralis cross-kingdom biofilms through pH buffering and biofilm suppression.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

BlOFP1 is associated with reduced secondary cell wall formation and interacts with BlKNOX9 and BlBLH9 in Betula luminifera.

Plant science : an international journal of experimental plant biology·2026
Same author

PmD479 is an Unutilized Gene for Powdery Mildew Resistance in Common Wheat.

Plant biotechnology journal·2026
Same author

<i>Nitrosomonas domestica</i> sp. nov. and <i>Nitrosomonas limosa</i> sp. nov., two ammonia-oxidizing bacteria from domestic wastewater and activated sludge.

International journal of systematic and evolutionary microbiology·2026
Same author

[Fe-EOB-tCDTA] generates strong contrast in the blood but not in the liver, despite inhibiting cellular [Gd-EOB-DTPA]<sup>2-</sup> uptake and partial liver excretion.

European radiology experimental·2026

相关实验视频

Updated: May 3, 2026

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
10:51

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Published on: December 5, 2016

9.7K

测序特征相关突变 (STAM) 来克隆耐基因

Fei Ni1, Yang Yu1, Lynn Epstein2

  • 1State Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China.

Methods in molecular biology (Clifton, N.J.)
|April 8, 2025
PubMed
概括

排序特征相关突变 (STAM) 是小麦的一种新型基因克隆方法. 这种方法简化了对特定特征负责的基因的识别,即使在复杂的植物基因组中也是如此.

关键词:
乙基甲硫酸盐 (EMS) 是一种基因克隆就是基因克隆.这是Iso-Seq.突变发生是突变发生的.在RNA-Seqq.抗性基因的抗性基因.这就是 STAM STAM 的意思.有条纹的生条纹的生

更多相关视频

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
11:37

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Published on: June 22, 2017

16.2K
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.6K

相关实验视频

Last Updated: May 3, 2026

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
10:51

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Published on: December 5, 2016

9.7K
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
11:37

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Published on: June 22, 2017

16.2K
Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
07:18

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

10.6K

科学领域:

  • 植物遗传学 植物遗传学
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 基因克隆对于了解植物特征至关重要.
  • 传统的方法可能很复杂,需要大量的资源,特别是对于小麦等多体物种.
  • 需要一种简化的方法来发现复杂基因组的植物中的基因.

研究的目的:

  • 介绍和详细介绍基因克隆的特征相关突变 (STAM) 测序方法.
  • 为实施STAM提供一个生物信息分析协议.
  • 为了突出STAM在其他植物物种中的适用性.

主要方法:

  • 使用野生类型植物的全长异形序列 (Iso-Seq) 作为参考.
  • 采用多个独立衍生突变体的转录组测序.
  • 专注于识别与特定特征相关的突变.

主要成果:

  • STAM方法绕过了对精细映射的需要.
  • 它不需要高质量的特定品种的全基因组组.
  • 该方法适用于复杂基因组的多种植物物种的基因克隆.

结论:

  • STAM为小麦基因克隆提供了一种简单而有效的方法.
  • 该方法强大,适用于具有复杂基因组结构的植物.
  • 为STAM数据的生物信息分析提供了详细的协议和提示.