相关实验视频

Updated: May 15, 2025

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

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学生和半专业翻译人员在翻译不同程度的翻译难度的文本时处理翻译单元

Fuxiang Wang1, Qingli Xu2

  • 1Center for College English Education Research, Qufu Normal University, Qufu, Shandong, Peoples' Republic of China.

PloS one
|April 8, 2025
PubMed
概括

翻译难度显著影响翻译人员如何处理文本单元 (TU). 更困难的文本导致更小的处理单元和增加非生产的TUs,无论翻译员的经验.

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Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

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相关概念视频

Initiation of Translation02:33

Initiation of Translation

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Translation01:31

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
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Termination of Translation01:44

Termination of Translation

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Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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