塑料介导的转化:一个新的航线,以导航等离子体传播的抗生素耐药性基因
Ifra Ferheen1, Lucia Cimarelli2, Stefania Marcheggiani3
1Laboratory of Genetics of Microorganisms and Microbial Biotechnology, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, MC, Italy; Department of Environment and Primary Prevention, National Institute of Health, 00161 Rome, Italy.
The Science of the total environment
|April 8, 2025
概括
在水生环境中的塑料碎片显著增强了细菌.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 生态毒理学 生态毒理学
背景情况:
- 塑料污染是水生生态系统日益关注的问题.
- 塑料作为细菌殖民和遗传交换的基质.
- 水平基因转移 (HGT) 是细菌适应的一个关键机制.
研究的目的:
- 研究塑料垃圾在细菌转化中的作用.
- 确定环境条件如何影响细菌吸收等离子体DNA.
- 评估塑料上抗生素耐药性基因的HGT潜力.
主要方法:
- 使用单个物种微观宇宙 (SSM) 和细菌联盟微观宇宙 (BCM) 模型.
- 暴露于不同的环境处理,包括塑料碎片中的大肠杆菌.
- 两个等离子体 (pACYC:Hyg和pBAV-1k) 的量化转换频率.
主要成果:
- 塑料碎片,特别是聚烯,显著增加了细菌对塑体DNA吸收的能力.
- 与塑料和塑料体同时化增强了应激生存的基因表达.
- 无论是SSM和BCM模型,都有效地模仿了自然转变事件.
结论:
- 塑料碎片作为水生环境中细菌转化的强有力的增强剂.
- 这一过程促进了抗生素耐药性基因通过HGT.传播.
- 微观宇宙模型是研究环境基因转移动态的宝贵工具.
相关概念视频
Antibiotic Selection
52.0K
Overview
52.0K
Genomic DNA in Prokaryotes
43.2K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.2K
Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K


