相关实验视频
Updated: May 15, 2025

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Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
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基因Xpert MTB/RIF Ct对于预测结核病传染性的有用性
F Méchaï1, E Beugre2, T Billard-Pomares3
1Infectious Disease Department, Avicenne Hospital, Hôpitaux Universitaires Paris Seine-Saint-Denis, AP-HP, Bobigny, France; IAME, INSERM UMR 1137, Université Paris Cité, Université Sorbonne Paris Nord, Paris, France.
Infectious diseases now
|April 8, 2025
概括
这是GeneXpert MTB/RIF测试.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断学
背景情况:
- 肺结核 (pTB) 诊断依赖于GeneXpert MTB/RIF,它比涂抹显微镜 (SM) 更敏感和更快.
- 估计患者的传染性对于控制结核病至关重要.
- 对于GeneXpert的循环值 (Ct) 对感染性评估的诊断实用性需要进一步评估.
研究的目的:
- 评估GeneXpert MTB/RIF测试的Ct值在估计肺结核患者的传染性方面的贡献.
- 为了比较GeneXpert测试的诊断准确性和传染性估计能力与传统方法,如涂抹显微镜和培养等.
主要方法:
- 在GeneXpert和培养方法确认的pTB患者身上进行了多中心的回顾性分析.
- 来自GeneXpert测试的循环值 (Ct) 值在0日 (D0) 记录.
- 分析了Ct值,涂抹显微镜等级,液体培养物的检测时间 (TTD) 和不同时间点 (D0,D15,月份1,月份2) 的培养阳性之间的相关性.
主要成果:
- 在426名患者中,30.6%的涂抹显微镜检查结果呈阴性.
- 在Ct值和涂抹显微镜等级之间观察到显著的相关性 (r = -0.77对于Xpert MTB/RIF,r = -0.69对于Xpert MTB/RIF Ultra).
- Ct值与培养的检测时间 (TTD) 有很强的相关性 (r = 0.71) 并预测D15.的阳性培养.
结论:
- GeneXpert Ct值是细菌负荷的可靠指标,与涂抹显微镜结果相对应得很好.
- 使用其Ct值的GeneXpert测试,可以潜在地取代涂抹显微镜用于诊断和传染性估计.
- 这种方法在资源有限的环境中尤为有价值,因为传统的培养和涂抹显微镜难以获得.
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