在伊拉克Al-Diwaniyah省的 brojlers中检测Eimeria tenella引起的分子检测和组织病理变化
Thanaa Ismael Jawad1, Ghaidaa Abass Jasim2
1Nursing Techniques Department, Technical Institute of Al-Dewaniyah, AL-Furat AL-Awsat Technical University, Najaf, Iraq.
Open veterinary journal
|April 9, 2025
概括
鸟类菌病是一种重要的家禽疾病. 这项研究使用显微镜和PCR在迪瓦尼亚省的中发现了Eimeria tenella,证实了它的存在和影响.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 禽类的健康 禽类的健康
- 分子诊断学 分子诊断
背景情况:
- 鸟类菌病对商业家禽产业构成了全球重大威胁.
- 这种疾病对全球的家禽健康和经济生存能力产生了重大影响.
研究的目的:
- 确定在迪瓦尼亚省负责子菌病的Eimeria物种.
- 通过分子方法确认Eimeria tenella的存在.
主要方法:
- 收集了150个鸟类样本,来自可疑患有菌病的.
- 利用显微镜,便漂浮和常规的聚合酶链反应 (PCR) 准18S rRNA基因.
- 进行部组织病理学检查.
主要成果:
- 显微镜分析检测到19.3%的便样本中有卵囊.
- 在22%的便样本中,PCR证实了Eimeria tenella.
- E. tenella的序列与GenBank记录有95%的相似性,表明了上皮损伤.
结论:
- 埃梅里亚天氏菌被证实是迪瓦尼亚省家禽种群中菌病的致病原体.
- 这些发现凸显了该地区E. tenella的流行率和病理影响.
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