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在基于比较转录组学的基础上,在绿色桃花,Myzus persicae (Hemiptera) 中识别了与RNAi效率相关的基因

Xiucheng Xie^1,2, Feng Shang^1,2, Yujing Liu^1,2

¹Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.

Pest management science

|April 9, 2025

概括

研究人员确定了影响绿色桃花的RNA干扰 (RNAi) 效率的关键基因. 这一发现旨在改善农业应用的基于RNAi的害虫控制策略.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

RNA干扰 (RNAi) 对虫控制有希望,但由于虫的低效率而面临局限性.
研究RNAi效率的遗传基础对于开发有效的害虫管理策略至关重要.

研究的目的:

为了确定调节绿色桃花,Myzus persicae.的RNAi效率的基因.
加强RNAi技术用于虫控制的实际应用.

主要方法:

使用了转录组分析和"RNAi的RNAi"方法.
人工饮食介导的dsrna传递被用来评估基因沉默和的产生.
权重基因共同表达网络分析 (WGCNA) 和差异基因表达 (DEG) 分析是在RNA-seq数据上进行的.

主要成果:

摄入dsMpHunchback和dsfusion dsRNA诱导了显著的基因沉默和减少了的产生.
编码基本区域白氨酸拉链 (gene11325) 和类似KIAA1161的葡萄糖酶 (gene6489) 的基因与增强的RNAi效率有关.
编码cathepsin B-like (gene12476) 和piggyBac可转移元素衍生蛋白3-like (gene7736) 的基因表现出补偿作用,抵消了RNAi.

结论:

鉴定了在Myzus persicae中调节RNAi效率的功能性作用的基因.
这些发现为优化基于RNAi的虫控制策略提供了基础.
突出了影响昆虫RNAi有效性的基因的功能多样性.

关键词:

在RNAi的效率上.在RNAi测定中的RNAi.比较的转录组学进行比较绿色桃花虫绿色桃花虫

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