在2018-2021年期间在科威特引起感染的Salmonella enterica血清型,通过多位置序列类型或全基因组测序来确定
Amani H Al Fadhli1, Wafaa Y Jamal2, Fatema Bibi Khodakhast2
1Department of Medical Laboratory Sciences, College of Allied Health Sciences, Kuwait University, Jabriya, Hawalli Governate, Kuwait.
Microbiology spectrum
|April 9, 2025
概括
这项研究在科威特发现了30种沙门氏菌血清型,其中S. Enteritidis和S. Typhimurium是最常见的. 全基因组测序揭示了一种独特的爆发菌株和S. Schwarzengrund的新序列类型,没有观察到对碳烯的抗性.
科学领域:
- 微生物学 微生物学
- 流行病学 流行病学
- 基因组学就是基因组学.
背景情况:
- 在全球范围内,非类型型沙门氏菌 (NTS) 感染具有独特的流行病学特征.
- 了解沙门氏菌血清型的多样性及其遗传构成对于公共卫生干预至关重要.
研究的目的:
- 在科威特确定人类Salmonella enterica分离的血清型和遗传多样性.
- 调查分离的遗传相关性,包括来自食物传播疫情的分离物.
- 评估孤立的抗生素敏感性模式.
主要方法:
- 多位点序列类型 (MLST) 用于确定序列类型 (ST) 和推断血清型.
- 全基因组测序 (WGS) 用于深入分析选定的分离物.
- 使用E-test.进行了抗生素敏感性测试.
主要成果:
- 总共有167个沙门氏菌分离体被确定,代表了30种不同的血清型.
- 沙门氏菌 (36.5%) 和沙门氏菌 (14.97%) 是主要的血清型.
- 一种新的序列类型,ST 10217 (Salmonella Schwarzengrund),是通过WGS发现的. 两个与疫情相关的S. Enteritidis分离物体表现出明显的遗传学特征. 没有检测到对碳烯的耐药性.
结论:
- 这项研究提供了有关沙门氏菌血清型在科威特引起人类感染的流行病学的重要数据.
- 这些发现有助于了解中东地区沙门氏菌的遗传多样性和抗菌素耐药性.
- 这些数据将有助于制定针对性策略来控制和治疗沙门氏菌病.
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