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Updated: Jul 3, 2026

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
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德克斯/上游程序用于雄羊精子选择

Rosaura Pérez-Pe1, Teresa Muiño-Blanco2, José Álvaro Cebrián-Pérez2

  • 1Grupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain. rosperez@unizar.es.

Methods in molecular biology (Clifton, N.J.)
|April 9, 2025
PubMed
概括

这项研究引入了一种新的德克斯/上游技术来分离精子,避免离心. 这种方法有效地恢复了高度运动和活力的雄羊精子,这对于生殖技术至关重要.

关键词:
选择 选择 选择 选择精液等离子体中的精液.分离 分离 分离 分离.精子动物 (Spermatozoa) 是一种精子.

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Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...