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相关概念视频

Downstream Processing01:29

Downstream Processing

Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...

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相关实验视频

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Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
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德克斯/上游程序用于雄羊精子选择.

Rosaura Pérez-Pe1, Teresa Muiño-Blanco2, José Álvaro Cebrián-Pérez2

  • 1Grupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain. rosperez@unizar.es.

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概括

这项研究引入了一种新的德克斯/上游技术来分离精子,避免离心. 这种方法有效地恢复了高度运动和活力的雄羊精子,这对于生殖技术至关重要.

关键词:
选择 选择 选择 选择精液等离子体中的精液.分离 分离 分离 分离.精子动物 (Spermatozoa) 是一种精子.

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科学领域:

  • 生殖生物学 生殖生物学
  • 精子分离 精子分离
  • 动物繁殖 动物繁殖

背景情况:

  • 精子与精液的分离对于生殖技术至关重要.
  • 传统方法通常涉及离心,这可能会损害精子,特别是公羊.
  • 精子受损会影响其运动性和活力,影响生殖成功.

研究的目的:

  • 开发一种非离心技术来分离雄羊精子.
  • 为了实现从精子等离子体中释放出运动和活性的精子的高恢复率.
  • 为一种简单,快速,高产量的精子分离方法提供详细的协议.

主要方法:

  • 开发了一种专门的右/上游技术,消除了离心的需要.
  • 描述了介质制备的详细协议和特定应用的修改程序.
  • 该方法需要最小的设备,并且可以适应各种哺乳动物物种.

主要成果:

  • 德克斯/上游技术成功地恢复了高度运动和活力的公羊精子.
  • 获得的精子没有来自精液等离子体,这表明有效分离.
  • 该程序被证明是简单的,快速的,高收益的.

结论:

  • 开发的右/上游技术为精子分离提供了离心技术的优越替代方案.
  • 这种方法保持了精子的运动性和活力,这对于辅助生殖技术至关重要.
  • 该技术广泛适用于对离心敏感的各种哺乳动物物种的精子.