Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.3K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Acrosomal Status and PARP-1 Nuclear Markers Could Improve Discrimination of Potential Fertility in Good-Quality Boar Semen Doses.

Reproduction in domestic animals = Zuchthygiene·2025
Same author

Antioxidant components of ram seminal plasma are influenced by age, season, and the RsaI polymorphism of the MTNR1A gene.

Animal reproduction science·2025
Same author

Dextran/Swim-Up Procedure for Ram Sperm Selection.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

From spermatogenesis to fertilisation: the role of melatonin on ram spermatozoa.

Domestic animal endocrinology·2025
Same author

Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

Animals : an open access journal from MDPI·2025
Same author

Spermiogram, Kinetics, Flow Cytometric Characteristics and DNA Damage Degree in Boar Ejaculates: Summarization and Clustering.

Veterinary sciences·2024

相关实验视频

Updated: May 15, 2025

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

20.2K

精子细胞内评估精子细胞内评估

Adriana Casao1, Sara Miguel-Jimenez2, Rosaura Pérez-Pe2

  • 1Grupo BIOFITER, Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón, Universidad de Zaragoza, Zaragoza, Spain. adriana@unizar.es.

Methods in molecular biology (Clifton, N.J.)
|April 9, 2025
PubMed
概括

本研究详细介绍了使用甲和染料评估精子细胞内的方法. 这些技术通过测量的分布和水平来评估精子电容和体反应.

关键词:
类四环环素的使用方法光显微镜显微镜是一种光显微镜.流动细胞计量流动细胞计量在Fluo-4-AM中使用.盘子阅读器光测量仪这是Rho-5-AM.

更多相关视频

Techniques for Imaging Ca2+ Signaling in Human Sperm
07:38

Techniques for Imaging Ca2+ Signaling in Human Sperm

Published on: June 16, 2010

17.0K
Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
05:44

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

8.1K

相关实验视频

Last Updated: May 15, 2025

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

20.2K
Techniques for Imaging Ca2+ Signaling in Human Sperm
07:38

Techniques for Imaging Ca2+ Signaling in Human Sperm

Published on: June 16, 2010

17.0K
Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
05:44

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

8.1K

科学领域:

  • 生殖生物学 生殖生物学
  • 细胞生理学 细胞生理学
  • 生物化学 生物化学

背景情况:

  • 细胞内 (Ca2+) 对精子功能至关重要,包括电容和体反应.
  • 准确测量Ca2+对于理解这些过程至关重要.

研究的目的:

  • 描述评估精子中Ca2+分布和水平的方法.
  • 为了将Ca2+动态与精子电容和体反应状态相关联.

主要方法:

  • 与精子容量相对的Ca2+分布的甲素 (CTC) 染色.
  • 使用低亲和力Rhod-5N-AM染料检测Ca2+水平的流细胞计.
  • 使用高亲和度Fluo-4-AM染料进行流细胞计,用于细胞内Ca2+变化.
  • 盘子阅读器光测量用于测量Fluo-4-AM装载精子中的Ca2+水平.

主要成果:

  • CTC有效地可视化了与精子容量相关的Ca2+分布.
  • 罗德-5N-AM允许在亚细胞区内对Ca2+进行评估.
  • -4-AM量化了细胞内Ca2+水平的变化.

结论:

  • 描述的方法为研究精子中的Ca2+提供了强大的工具.
  • 这些技术对研究精子电容和体反应有价值.
  • 不同的染料亲和度可以针对性地研究Ca2+动态.