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减少表示双硫酸盐测序库精子DNA甲基化分析的准备:手动和自动化方法

Jean-Philippe Perrier^1,2, Aurélie Chaulot-Talmon^1,2, Francesca Ali^1,2

¹Université Paris-Saclay, UVSQ, INRAE, BREED, Jouy en-Josas, France.

Methods in molecular biology (Clifton, N.J.)

|April 9, 2025

概括

使用减少表示二硫酸盐测序 (RRBS) 进行精子DNA甲基化分析的自动化提高了其性能和可重复性. 这一进步有助于理解生育能力和精子质量研究.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
生殖生物学生殖生物学
基因组学就是基因组学.

背景情况:

精子中的DNA甲基化是与生育能力和精子质量相关的关键表观遗传修饰.
减少表示双硫酸盐测序 (RRBS) 是研究精子DNA甲基化的一个有价值的工具.
目前的RRBS图书馆准备协议是劳动密集型的,容易出现技术变化.

研究的目的:

为了提高精子DNA甲基化分析的性能和可重复性,使用RRBS.
解决与手动RRBS库编制相关的技术挑战.

主要方法:

已建立的RRBS协议的调整.
适应协议在汉密尔顿管道自动机上实现,用于自动化图书馆准备.

主要成果:

自动化的RRBS协议显示性能有所改善.
通过自动化实现了精子DNA甲基化概况的增强可复制性.

结论:

自动化RRBS库准备是一个可行的策略,以提高效率和一致性.
这种优化的方法可以促进对精子表观遗传学及其对生殖结果的影响进行更可靠的研究.

关键词:

自动化自动化自动化通过DNA甲基化.全基因组表观遗传分析高通量的高通量.减少表示双硫酸盐测序.精子精子精子

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