仪器复合:扩大吞吐量测量维生素B1在全血使用平行液态染色学系统与单个质谱仪接口的扩大吞吐量
Preejith P Vachali1, Erik Kish-Trier1, Sophie Steckel1
1Mass Spectrometry Department, ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, United States.
Clinical chemistry
|April 9, 2025
概括
一种新的LC-MS/MS方法准确地测量全血中的二胺二酸盐 (TDP). 这种高通量测定提高了维生素B1测试的效率,这对于评估营养状况至关重要.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 胺二酸盐 (TDP) 是维生素B1的活性形式,对于细胞能量代谢至关重要.
- 营养状况的临床评估依赖于TDP分析,特别是对于有缺陷风险的个体.
- 越来越多的维生素B1测试需求需要高通量分析方法.
研究的目的:
- 开发和验证一种高通量液体染色学-并联质谱法 (LC-MS/MS) 方法,用于在全血中量化TDP.
- 为多重LC-MS/MS平台适应快速样品制备技术.
主要方法:
- 全血样被用三酸去蛋白化,并加上同位素标记的TDP (TDP-d3).
- 使用逆相色谱分离TDP,并通过阳性电喷射电离化LC-MS/MS分析.
- 一个由4个LC仪器连接到单个质谱仪的系统使分阶段注射和快速吞吐量成为可能.
主要成果:
- 该方法证明了从20到1000nmol/L的线性,精度为±15%,不精确度为≤3%.
- 观察到优异的跨流平价 (<2%的不精确度),没有转移.
- 该平台在4.5小时内产生了81个结果,大大减少了质谱仪置时间.
结论:
- 成功开发了一种经过验证,准确,特定和高通量LC-MS/MS的全血TDP测量方法.
- 简单,快速的样品制备和分阶段注射策略提高了分析效率.
- 这种方法有效地解决了对维生素B1检测的日益增长的需求.
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