使用亚临界水从花中绿色提取异黄:动力学,优化和与其他以水为基础的可持续方法的比较
Pedro Barea1, Alba Ester Illera1, Rodrigo Melgosa1
1Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos. Spain.
Food chemistry
|April 9, 2025
概括
亚临界水有效地从草中提取异黄,产生最高度的生物可用形式. 其他方法,如微波和超声波提取也被探索进行比较.
科学领域:
- 食品科学 食品科学 食品科学
- 绿色化学 绿色化学
- 生物技术是生物技术.
背景情况:
- 奥卡拉是大豆的副产品,富含异黄,有价值的生物活性化合物.
- 传统的提取方法往往使用苛刻的溶剂,促使人们研究更绿色的替代品.
- 异黄以各种形式存在,其中糖化物,马洛尼尔糖化物和亚格利康具有不同的生物利用性.
研究的目的:
- 为了评估亚临界水 (subW) 作为绿色提取溶剂的异黄从okara.
- 为了比较subW提取与微波辅助提取 (MAE) 和超声波辅助提取 (UAE) 的效率.
- 分析温度和时间对异黄产量和形式的影响.
主要方法:
- 在洗过的 (OKW) 和未预处理 (OKC) 上进行了亚临界水提取.
- 微波辅助提取 (MAE) 和超声波辅助提取 (UAE) 用于比较.
- 进行了动态分析,以了解异黄的相互转化和降解.
主要成果:
- 亚临界水提取从OKC在120°C30分钟的温度下从OKC中获得的最多1229μg异黄/g干.
- 基因斯坦家族是最丰富的异黄组.
- 马埃和阿联分别实现了72.8%和75.4%的亚W产量,但亚W产生了更高的生物可用β-糖化物和糖形式.
结论:
- 亚临界水是一种有前途的绿色溶剂,用于从okara.com提取异黄.
- 提取条件显著影响异黄产量和生物可用性.
- 与MAE和UAE相比,亚临界水提取有利于生产更多可生物利用的异黄形式.
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