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Updated: Jun 23, 2026

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP
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Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP

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分子相互作用的综合生物物理特征:用sfGFP-nanobody复合物的案例研究

Aysha K Demeler1, James Bosco2, Matthew J Sydor3

  • 1Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, AB, T1K 3M4, Canada.

Analytical biochemistry
|April 9, 2025
PubMed
概括

这项研究比较了蛋白质与蛋白质相互作用 (PPI) 的分析生物物理方法. 结合多种技术,可以更完整,更可靠地描述溶液中的PPI.

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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

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Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay14:34

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...