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Updated: May 15, 2025

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Published on: May 22, 2018

动物性多宿主寄生虫Schistosoma japonicum中抗原多样性的演变:对疫苗设计的影响

Daniel A J Parsons¹, Anthony J Walker², Aidan M Emery³

¹Molecular Parasitology Laboratory, School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Kingston Upon Thames, Penrhyn Road, Surrey, England KT1 2EE, United Kingdom; Natural History Museum, Cromwell Road, South Kensington, London, England SW7 5BD, United Kingdom.

International journal for parasitology

|April 9, 2025

概括

在Schistosoma japonicum抗原基因中的遗传多样性因宿主而异,由免疫压力和动物传播驱动. 这影响了用于控制杆菌病的疫苗开发.

科学领域:

寄生虫学的寄生虫学
进化生物学进化生物学
免疫学免疫学免疫学

背景情况:

动物传染病日本杆菌,面临消除挑战,由于复杂的多宿主传染的日本杆菌.
多宿主传播对S. japonicum遗传多样性和抗原编码基因 (SjACG) 变异性的影响尚不清楚.
了解SjACG的变异性对于寄生虫的生存,疫苗开发和疾病控制策略至关重要.

研究的目的:

在中国的不同确定的宿主物种中分析关键的Schistosoma japonicum抗原编码基因 (SjACGs) 的遗传多样性.
研究宿主免疫选择压力对SjACG变异的影响.
评估动物传播在SjACG多样性传播中的作用.

主要方法:

来自S. japonicum miracidia的三个显著SjACG (TSP-23,VAL-7,TAL-1) 的宿主相互作用域的测序. 在FTA卡片上存档.
在中国大陆的各种最终宿主物种中,从自然感染中抽取寄生虫的样本.
对SjACG单双型多样性和宿主物种之间的分布进行分析.

主要成果:

在宿主物种中观察到SjACGs的高遗传多样性,每个基因确定了10-20种类型.
抗原单元类型在宿主物种中普遍存在,这表明了基因流动和动物传播的重要性.
来自人类的寄生虫表现出最高的SjACG多样性,与所有其他宿主物种共享单元型,表明宿主特异性适应和快速多样化.

结论:

动物传播对推动S. japonicum抗原变异的共同进化过程产生重大影响.
宿主衍生的免疫选择压力可能驱动SjACG多样化,影响抗原结构,功能和抗原性.
这些发现为适应性分子进化提供了洞察力,并有助于开发有效的抗瘤疫苗和控制动物性感染性瘤病.

关键词:

抗原编码基因的基因.多主机传输多主机传输 schistosome抗原变异的变化这种疫苗是schistosome疫苗.这是SjTSP-23,SjVAL-7,SjTAL-1.动物性传染性石杆菌病.

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