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在人类支气管平滑肌细胞中,基因组胺诱导的细胞溶动员
Kyung Jin Choi1, Woo Young Jeon2, Mee Young Lee2
1Department of Physiology, College of Medicine and Myunggok Medical Research Institute, Konyang University, Daejeon 35365, Korea.
概括
组胺通过H1受体在人类支气管平滑肌细胞中调动,激活脂酶C/异醇三酸盐通路. 线粒体也在这种释放机制中发挥作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 胰岛素是已知的支气管收缩的调解者.
- 支气管平滑肌细胞中由组胺诱导的动员的精确机制尚未完全理解.
研究的目的:
- 在培养的人类支气管平滑肌细胞中研究对调动的组胺作用机制.
- 为了阐明参与基因组胺诱导的释放的信号通路.
主要方法:
- 在人体支气管平滑肌细胞培养上进行了体外成像实验.
- 实验使用了各种抑制剂,包括H1受体对抗剂,脂酶C抑制剂和内醇三酸盐受体抑制剂.
- 使用特定的抑制剂和再 perfusion 协议来评估线粒体功能.
主要成果:
- 希斯胺以度依赖的方式增加了细胞内水平 (EC50 = 3.00 ± 0.25 μM).
- 的释放来自细胞内储存,独立于细胞外.
- 组胺诱导的升被H1受体对抗剂,PLC抑制剂 (U73122) 和InsP3受体抑制剂 (咖啡因) 阻断.
- 线粒体抑制剂 (FCCP,奥利戈米辛) 减弱了基因组胺诱导的释放,并在再注射时抑制了升.
结论:
- 组胺激活H1受体,导致脂酶C (PLC) 和伊诺西1,4,5-三酸盐 (InsP3) 途径的激活.
- 这一途径通过人类支气管平滑肌细胞中的InsP3受体从细胞内储存的中调动.
- 线粒体参与了由组胺诱导的细胞内释放过程.
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