随着时间的推移,转录组和染色质可访问性分析揭示了动态转录性调节,塑造了尖端的船体大小
Shaotong Chen1, Fuquan Li1, Weizhi Ouyang2
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
米粒的发育受转录因子的调节,如生长调节因子和SCL6-IIb,影响谷粒大小和产量. 在关键基因中编辑cis-regulatory元素可以提高粒度的长度.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学 是一个
- 农业科学 农业科学
背景情况:
- 米 (Oryza sativa) 的螺纹体决定了谷物储存能力,影响了产量和质量.
- 了解转录调节对于改善米粒发育至关重要,因为目前的知识有限.
研究的目的:
- 描述在米尖皮发育期间的转录和染色质可访问性动态.
- 为了确定控制大米粒大小的关键转录调节器和cis调节元素.
主要方法:
- 用RNA-sequencing (RNA-seq) 和ATAC-sequencing (ATAC-seq) 来进行时间过程分析.
- 基因表达和染色质可访问性与发育阶段相关.
- 使用CRISPR-Cas9编辑来修改开放色素区域 (OCR).
主要成果:
- 超过一半的基因在船体发育过程中呈现出序列表达,大多数OCR的变化中度.
- 发现增长调节因子 (GRF) 通过影响代谢和信号通路,对谷粒大小至关重要.
- 甲状腺促进剂结合蛋白类型18 (OsSPL18) 激活SCL6-IIb,这是一个调节谷粒大小的GRAS家族转录因子.
- 在BRASSINAZOLE-RESISTANT 1 (BZR1) 和SCL6-IIb的上游编辑OCR导致了更长的粒度.
结论:
- 已经阐明了大米谷物产能的全面cis-regulatory景观.
- GRF和SCL6-IIb在米谷物发展中发挥着重要作用.
- 针对性地编辑OCR是一个有前途的策略,可以提高米粒大小.
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