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CsMYB1-CwINV6模块涉及在干旱压力下促进类水果中可溶性糖的积累
Muhammad Abbas Khan1, Fatima Zaman1, Yong-Zhong Liu1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
Plant, cell & environment
|April 10, 2025
概括
干旱通过提高水槽强度来增加水果糖. CsMYB1转录因子直接激活CwINV6的表达,增强类水果中可溶性糖的积累.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 干旱压力通过改变可溶糖含量,影响水果质量.
- 细胞壁逆转酶 (CwINV) 对于调节糖分和水果水槽强度至关重要.
- 在干旱期间类植物CwINV6的精确转录调节在很大程度上是未知的.
研究的目的:
- 研究MYB转录因子CsMYB1在干旱期间调节类水果糖累积中的作用.
- 阐明将CsMYB1与CwINV6表达和干旱反应联系起来的分子机制.
主要方法:
- 描述CsMYB1基因及其亚细胞局部.
- 在干旱和果中ABA治疗下分析CsMYB1和CwINV6转录水平.
- 使用短暂过度表达和病毒诱导的基因沉默 (VIGS) 的功能分析.
- 酵母一杂交 (Y1H) 和 luciferase 记者测定以确认促进体结合和转录激活.
主要成果:
- 在干旱和ABA治疗后,CsMYB1和CwINV6的表达显著增加.
- 过度表达CsMYB1或CwINV6增强了果中的可溶糖积累 (葡萄糖和果糖).
- 制 CsMYB1 或 CwINV6 降低可溶糖含量.
- CsMYB1直接与CwINV6促进体结合,并积极调节其表达.
结论:
- CsMYB1-CwINV6模块在类水果中干旱引起的可溶糖积累中发挥着关键作用.
- 干旱通过CsMYB1-介导的CwINV6.6激活增强了水果沉降强度.
- 这项研究揭示了一条关键的监管途径,以改善水资源有限的水果质量.
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