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Updated: Jun 20, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

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设计一个远端部位和测试-代并行突变的工程氧类固醇脱酶的设计

Yuan Li1,2, Shu-Fang Li1,2, Sen-Yu Fu1,2

  • 1Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

Journal of agricultural and food chemistry
|April 10, 2025
PubMed
概括

使用远端位点和测试-代并行突变发生的蛋白质工程 (DSST-IPM) 创建了一个高度活性和热稳定的酸脱酶. 这一策略提高了工业生物催化剂的酶性能.

关键词:
7β-基类固醇脱酶.远端蛋白质工程 远端蛋白质工程远端部位和测试试验氧化类固醇脱基酶.进行并行代查.

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A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

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相关概念视频

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide02:44

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.