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对瘤-肌瘤边界细胞特征的空间多组学分析,用于预测乳腺癌的进展和治疗反应

Yuanyuan Wu¹, Youyang Shi¹, Zhanyang Luo²

¹Department of Breast Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Frontiers in cell and developmental biology

|April 10, 2025

概括

乳腺癌瘤边界是影响进展和治疗的复杂区域. 向与癌症相关的纤维细胞和M2巨细胞可能会改善结果,新的恶性体征预测预后不佳.

科学领域:

在瘤学瘤学.
癌症生物学癌症生物学
空间转录学空间转录学

背景情况:

乳腺癌的瘤边界是一个异质的区域,恶性和非恶性细胞相互作用影响瘤进展,免疫逃避和耐药性.
乳腺癌瘤边界的空间转录形状和预后意义尚不清楚.

研究的目的:

为了研究乳腺癌瘤边界的空间转录特征.
识别瘤边界内的关键细胞与细胞相互作用和分子特征.
评估乳腺癌瘤边界的预后价值和治疗影响.

主要方法:

利用Cottrazm算法进行边界重建和差异表达基因 (DEGs) 的识别.
采用SpaCET进行细胞-细胞共定位分析,揭示瘤相关巨细胞 (TAM) 和癌症相关纤维细胞 (CAF) 之间的相互作用.
开发并验证了使用拉索回归和TCGA数据进行预后和治疗响应评估的恶性身体特征 (MBS).

主要成果:

瘤边界的特点是细胞外矩阵重建,免疫调节调节,以及上皮转移到介质酶转移 (EMT).
观察到CAFs和M2型TAMs之间的显著相互作用,与免疫排除和耐药性相关.
高MBS得分与较差的生存率和减少对化疗的反应有关,表明其预后和预测价值.

结论:

乳腺癌瘤边界的空间转录揭示了瘤进展和治疗抵抗中的关键分子参与者.
准CAF-M2表型相互作用是克服免疫抑制和增强治疗反应的潜在策略.
乳腺癌系统作为一种新的预后工具,用于分层乳腺癌患者,并指导个性化治疗策略.

关键词:

这就是CAF-M2相互作用的原因.乳腺癌乳腺癌乳腺癌预测模型的预测模型.空间转录学空间转录学治疗耐药性治疗耐药性瘤的边界是瘤的边界.

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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...