隔离,植物遗传学和新PDCoV菌株的特征,该菌株会影响人类细胞中的细胞基因表达
Xiaozhu Yang1, Hanwei Yin1, Mengyuan Liu1
1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.
Frontiers in microbiology
|April 10, 2025
概括
一种新的猪三角型冠状病毒 (PDCoV) 菌株被分离和分析. 人体细胞中PDCoV感染会触发免疫反应,自和铁,从而提供了对其机制的见解.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 猪三角型冠状病毒 (PDCoV) 在猪肉行业造成重大经济损失,原因是严重的小猪病.
- PDCoV的广泛宿主范围,包括潜在的人类传播,引发了公共卫生方面的担忧.
- 关于PDCoV流行,特征和宿主基因表达的理解有限.
研究的目的:
- 为了分离和描述一个新的PDCoV菌株.
- 在人类肝瘤 (Huh7) 细胞中研究由PDCoV感染引起的宿主细胞基因表达变化.
- 阐明PDCoV病原发生的基础分子机制.
主要方法:
- 一个新型PDCoV菌株 (CHN/SX-Y/2023) 的隔离和鉴定.
- 对受感染的Huh7细胞进行了植物遗传学分析和转录组分析.
- 对差异表达基因 (DEG) 和信号通路的分析.
主要成果:
- 新的PDCoV菌株属于中国血统,在敏感细胞系中表现出细胞病变效应.
- 转录组分析显示,PDCoV感染的Huh7细胞中有1799个上调调节的和771个下调调节的基因.
- 上调基因与免疫/炎症反应和脂质代谢有关;丰富的途径包括MAPK,TNF和NF-κB.
结论:
- 在Huh7细胞中,PDCoV感染会诱导内在免疫反应,细胞自和铁亡.
- 确定了与炎症和免疫相关的关键基因.
- 这些发现为PDCoV感染特征和机制提供了新的见解.
相关概念视频
Plant Tissue Culture
33.3K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
33.3K
Plant Breeding and Biotechnology
17.2K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
17.2K
Transgenic Plants
7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K
Conjugation
3.0K
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
3.0K
Microbe-Plant Interactions
140
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
140


