CCFM1376通过调节胆汁酸的组成来改善小鼠的高胆固醇血症
Kexue Chen1,2, Danting Dang1,2, Huizhen Li1,2
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, Jiangsu, China.
Microbiome research reports
|April 10, 2025
概括
乳酸菌约翰尼 (Lactobacillus johnsonii) CCFM1376通过改变胆酸代谢,有效地降低了高胆固醇贫血小鼠的胆固醇. 这种益生菌菌株为管理胆固醇水平提供了潜在的策略.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 胃肠病学 胃肠病学
背景情况:
- 在某些细菌菌株中,高胆汁盐化酶 (BSH) 活性表明胆固醇调节的潜力.
- 高胆固醇血症仍然是一个重大的全球健康问题,需要新的治疗策略.
研究的目的:
- 评估Lactobacillus johnsonii CCFM1376在高胆固醇血症小鼠模型中的降胆固醇效应.
- 阐明涉及L. johnsonii CCFM1376.7的胆酸代谢调节的潜在机制.
主要方法:
- 使用ninhydrin方法量化BSH活性.
- 在C57BL/6J小鼠中,通过高胆固醇饮食诱导高胆固醇血量,随后进行8周的L. johnsonii CCFM1376.6.
- 血清和肝脏的生物化学,胆汁酸概况 (LC-MS) 和胆汁酸代谢相关基因的胆汁和肝脏的基因表达分析 (qPCR) 被执行.
主要成果:
- L. johnsonii CCFM1376显著降低了血清总胆固醇和LDL-C,同时增加了HDL-C水平.
- 在CCFM1376治疗组中,肝胆固醇水平也显著下降.
- 治疗导致肝肠循环中非结合胆酸的增加和便胆酸分泌的增加,以及改变叶FXR,FGF15和CYP7A1的表达.
结论:
- 牛乳杆菌约翰尼 CCFM1376 在改善小鼠高胆固醇血症方面表现出有效性.
- 降胆固醇的作用是通过调节胆酸代谢和成分来调节的.
- 这项研究支持使用L. johnsonii CCFM1376等益生菌作为管理胆固醇疾病的潜在策略.
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