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Updated: May 15, 2025

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

一个双阶段的调节机制动态控制与生长相关的促进体上由SAGA复合体对 histone H3 的乙化

Sevil Zencir¹, Daniel Dilg¹, Maria Jessica Bruzzone¹

¹Department of Molecular and Cellular Biology, Université de Genève, 1211, Geneva, Switzerland.

Nucleic acids research

|April 10, 2025

概括

压力通过改变基因素H3 lysine 9乙化 (H3K9ac) 来降低与生长相关的基因的调节. 这个过程涉及转录激活剂和抑制剂的动态结合,独立于转录,以控制基因表达.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因规则基因规则

背景情况:

基因组H3氨酸9乙化 (H3K9ac) 与活性基因转录有关.
连接H3K9ac动态与基因表达变化的精确机制,特别是在压力期间,尚未完全理解.
现有知识在很大程度上是相关的,需要机理学研究.

研究的目的:

研究H3K9ac与酵母在压力条件下的基因表达之间的关系.
为了确定在压力期间由H3K9ac调节的特定基因.
阐明转录激活剂,抑制剂,SAGA和RNA聚合酶II在H3K9ac调控中的作用.

主要方法:

在酵母菌中分析与生长相关 (GR) 基因.
在压力时监测基因转录和H3K9ac水平.
研究转录激活剂和抑制剂的结合动态.
评估Spt-Ada-Gcn5乙转移酶 (SAGA) 和RNA聚合酶II的参与.

主要成果:

确定了一组GR基因,在压力下显著降低转录和H3K9ac的调节.
证明了H3K9ac调节的两步机制,涉及转录抑制剂和激活剂的动态结合.
表明H3K9ac的变化独立于活性转录.
发现转录激活剂的释放先于H3K9ac的减少,而抑制剂结合则建立了抑制.

结论:

H3K9ac的动态被一个转录独立的机制主动指挥,该机制涉及转录因子的顺序结合.
压力诱导的GR基因降低调节是由激活剂释放的媒介,其次是抑制剂结合,导致H3K9ac的减少和基因沉默.
这项研究提供了关于H3K9ac等表观遗传标记如何被动态调节以控制环境压力期间的基因表达的机制性见解.

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