Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Photoluminescence: Applications01:14

Photoluminescence: Applications

343
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
343

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Simultaneous Capture and Luminescent Detection of Trace Benzene in Cyclohexane Enabled by π-Accepting Benzothiadiazole Units in a Metal-Organic Framework.

Journal of the American Chemical Society·2026
Same author

Mixed-Lanthanide Metal-Organic Frameworks for Near-Infrared-I Bioimaging and Near-Infrared-III Four-Photon Upconversion.

Journal of the American Chemical Society·2026
Same author

Correction: Confinement of Cd(II) by 2,2'-bipyridyl: control of structural transformations and porosity in perfluorinated biphenyldicarboxylate MOFs.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Modulating Room-Temperature Phosphorescence by Coordination Interactions and Structural Rigidity in Dual-Ligand MOFs.

Inorganic chemistry·2026
Same author

Confinement of Cd(II) by 2,2'-bipyridyl: control of structural transformations and porosity in perfluorinated biphenyldicarboxylate MOFs.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Three-Dimensional Chiral Metal-Organic Frameworks: Synthesis and Structural Transformations.

Nanomaterials (Basel, Switzerland)·2026

相关实验视频

Updated: May 15, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.0K

灵敏的辐射测量发光FRET探头用于检测黄素.

Wei Liu1, Wei Yao1, Andrei S Potapov2

  • 1Liaoning Province China-Russia Magnesium Materials Belt and Road Joint Laboratory, School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, Liaoning, P. R. China.

Inorganic chemistry
|April 10, 2025
PubMed
概括

新的金属有机框架 (MOF) 作为对利博黄素 (RF) 的敏感传感器. Ho-MOF在真实样本中提供了出色的抗干扰,快速检测和实际应用,证明了射频监控的巨大潜力.

更多相关视频

FRET Imaging in Three-dimensional Hydrogels
09:47

FRET Imaging in Three-dimensional Hydrogels

Published on: August 1, 2016

13.0K
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.0K

相关实验视频

Last Updated: May 15, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.0K
FRET Imaging in Three-dimensional Hydrogels
09:47

FRET Imaging in Three-dimensional Hydrogels

Published on: August 1, 2016

13.0K
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.0K

科学领域:

  • 材料化学 材料化学
  • 分析化学 分析化学
  • 纳米技术纳米技术

背景情况:

  • рибофлавин (RF) 是一种重要的营养素,其准确的量化对健康和食品质量至关重要.
  • 开发选择性和敏感的射频检测方法仍然是分析化学的一个重大挑战.
  • 金属有机框架 (MOF) 为先进的传感应用提供可调节的特性.

研究的目的:

  • 为了合成新型异构的2DMOFs用于利博弗拉的发光感应.
  • 研究合成的MOF的传感性能,包括选择性,灵敏性和稳定性.
  • 探索开发的基于MOF的传感器在现实生活样本中的实际应用性.

主要方法:

  • 两种同型二维MOF的溶热合成:Ho-MOF和Er-MOF.
  • 使用弗斯特共振能量转移 (FRET) 进行比率发光感应的利博黄素.
  • 通过反干扰测试,pH稳定性,响应时间和循环检测来评估传感器性能.

主要成果:

  • 无论是Ho-MOF还是Er-MOF,都表现出优异的抗干扰能力和RF的低检测极限 (Ho-MOF的低至1.72nM).
  • 霍-MOF表现出优越的性能,具有增强的抗干扰,低检测极限 (1.72 nM),出色的pH稳定性 (4-12) 和快速响应 (<50秒).
  • 霍-MOF在尿液和血清等真实样本中显示出实际适用性,检测极限分别为5.26nM和27.27nM,VB2片中的恢复率高.

结论:

  • 合成的Ho-MOF和Er-MOF是基于FRET机制的利博弗拉的有效发光传感器.
  • 霍-MOF表现出卓越的传感特性,使其成为实用,选择性和敏感的 рибофлавин检测的有希望的候选人.
  • 这项研究突出了MOF在开发先进分析工具的潜力,用于监测各种环境中的重要化合物.