相关实验视频

Updated: May 23, 2025

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

使用复合抗体基受体的A型流感病毒的细胞结合,吸收和感染

Oluwafemi F Adu¹, Milagros Sempere Borau², Simon P Früh^1,3

¹Department of Microbiology and Immunology, College of Veterinary Medicine, Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA.

Journal of virology

|April 10, 2025

概括

研究人员设计了一种新型蛋白质受体,以研究流感A病毒如何进入细胞. 这种新受体结合病毒并促进其吸收,模仿自然感染途径以进行更好的研究.

科学领域:

病毒学和分子生物学
细胞和分子免疫学细胞和分子免疫学

背景情况:

甲型流感病毒利用酸 (Sia) 受体通过内细胞突变进入细胞,但Sia载体和受体-病毒相互作用的确切作用尚不清楚.
了解流感病毒结合和随后进入细胞的机制对于开发有效的抗病毒策略至关重要.

研究的目的:

设计和验证一种工程蛋白质受体,用于研究流感A病毒 (H1亚型) 的结合和细胞进入过程.
在病毒感染的背景下,探索受体结合的功能作用,而不仅仅是简单的附着.

主要方法:

通过将抗血凝素 (HA) 抗体可变域 (scFv) 与猫类转移素受体1型 (fTfR) 序列融合而构建了一个工程受体.
这种受体因遗传淘汰 (Slc35A1) 而表达在缺乏内源性Sia的细胞中.
评估了病毒结合,内细胞分裂和感染水平,以及克拉中介内细胞分裂抑制剂 (CME) 的影响.

主要成果:

设计的受体定位在细胞表面,有效地结合H1流感病毒和HA蛋白,并调解有效的病毒内细胞分裂.
感染水平与表达Sia细胞中的水平相似,低亲和感受体突变体显示出增强的感染.
抑制CME显著降低了病毒的进入,证实了与Sia依赖的入口使用的类似的内化途径.

结论:

基于抗体的受体提供了一个新的工具来剖析流感病毒与宿主细胞的相互作用和进入机制.
该系统允许对受体结合在病毒感染中的作用进行受控调查,独立于内源性Sia.
这些发现凸显了工程受体在研究病毒进入途径和开发新治疗点方面的实用性.

关键词:

细胞内分泌症 (endocytosis) 发生在细胞内.实验工具实验工具实验工具主体细胞的相互作用.这种病毒是orthomyxovirus.结合受体与受体的结合方式

更多相关视频

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

Published on: May 29, 2016

Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry

Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry

Published on: November 4, 2015

相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...