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在Cryptococcus neoformans粘附和感染大脑内皮细胞中CD146的参与

Wei Liu¹, Junhong Chen¹, Ting Wang¹

¹Department of Immunology, National Vaccine Innovation Platform, NHC Key Laboratory of Antibody Technique, Jiangsu Key Laboratory of Pathogen Biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu, China.

Infection and immunity

|April 10, 2025

概括

CD146是一种大脑内皮细胞粘附分子,会影响Cryptococcus neoformans的结合,但不会显著影响小鼠的大脑感染或生存,这表明它在中枢神经系统入侵中起着不可或缺的作用.

科学领域:

神经科学是一个神经科学.
传染性疾病传染性疾病
免疫学免疫学免疫学

背景情况:

加密球菌脑膜炎是一种严重的中枢神经系统 (CNS) 感染,由Cryptococcus neoformans引起.
菌进入中枢神经系统需要突破血脑屏障 (BBB),包括对大脑微血管内皮细胞 (BMEC) 的粘附.
CD146是一种粘附分子,表达在BMEC上,可能调解真菌与BBB的相互作用.

研究的目的:

调查CD146在Cryptococcus neoformans中扮演的角色,对BBB的粘附和入侵.
确定CD146调制对真菌中枢神经系统感染和宿主生存的影响.

主要方法:

在C. neoformans感染后的BMEC中检查了CD146表达.
在体外利用CD146过度表达和淘汰模式来评估真菌粘附.
在体内给予抗CD146抗体 (AA98),以评估感染小鼠对真菌病和生存的影响.

主要成果:

新型菌感染减少了BMEC上CD146的表达,部分是通过金属蛋白酶-9.
过度表达的CD146在体外增强了真菌对BMECs的附着性,而CD146淘汰则减少了它.
CD146淘汰并没有减少真菌性脑部感染,但抗CD146抗体增加了真菌病并没有改善存活率.

结论:

CD146参与C. neoformans对大脑血管的初始粘附.
尽管影响了早期粘附,但CD146似乎在已建立的C. neoformans脑部感染和整体宿主生存中发挥了不可或缺的作用.

关键词:

CD146 是一个 CD146 是一个 CD146 这是Cryptococcus neoformans的新型菌.血脑屏障是什么意思内皮细胞是内皮细胞.中和化中和化中和化

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