凝聚素和凝聚素的参与规则指导了线粒体染色体的形成
Kumiko Samejima1, Johan H Gibcus2, Sameer Abraham3
1Institute of Cell Biology, University of Edinburgh, Edinburgh, UK.
概括
染色体的结构维护 (SMC) 复合物如凝聚素和凝聚素可以调节染色体的折叠. 在分裂过程中,冷凝素分解凝聚环并保持姐妹染色体凝聚力.
科学领域:
- 细胞生物学
- 分子生物学
- 遗传学
背景情况:
- 染色体组织对于基因组功能至关重要.
- 染色体结构维护 (SMC) 复合体,包括凝聚素和凝聚素,在染色体结构中发挥关键作用.
- 了解这些复合体如何相互作用形成线粒染色体是必不可少的.
研究的目的:
- 在从互相转向线粒染色体的过程中定义管理SMC复杂相互作用的规则.
- 阐明凝聚素和凝聚素在染色体组织和姐妹染色体凝聚中的不同作用.
主要方法:
- 这里是H-C
- 图像制造
- 蛋白质组学
- 聚合物建模
主要成果:
- 凝聚素通过移位凝聚素来拆解相间染色蛋白循环.
- 凝聚蛋白绕过凝聚蛋白以保持姐妹染色体凝聚力.
- 线粒染色体模型显示连续的,不重叠的循环,表明凝结物在遇到时会停滞.
- 在体内循环挤出由凝聚物发生在1-3kb/s,形成螺旋式支架.
结论:
- 凝聚素和凝聚素在线粒染色体形成中具有不同的顺序作用.
- 凝聚素积极重塑色素,而凝聚素则被移除,但保持凝聚力.
- 在细胞分裂过程中,SMC复合体的动态决定了染色体结构.
更多相关视频
12:26Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
18.5K
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
14.2K
相关概念视频
Condensins
3.2K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
3.2K
Cohesins
4.2K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
4.2K
Forces Acting on Chromosomes
3.3K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
3.3K
Attachment of Sister Chromatids
3.0K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules. Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.0K
Separation of Sister Chromatids
3.5K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.5K
Chromosome Structure
22.4K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.4K
