常见的血液采样类型对代谢查的影响
Ceylan Bal1,2, Esra Özyurt2, Gülsen Yılmaz1,2
1Department of Biochemistry, Ankara Yıldırım Beyazıt University, Ankara, Turkey.
概括
用于代谢查的干血点检测显示,在使用各种血液采集管时,与毛细血管全血相比,氨基酸和甲酸水平存在显著差异. 结果因采样方法而异,影响测试解释.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 质谱测量质量谱测量
背景情况:
- 毛细血管血液经常用于代谢查中的干血点 (DBS) 样本.
- 血液采集方法的变化可能会影响DBS测试中的分析物度.
- 样本采集的标准化对于准确的代谢查结果至关重要.
研究的目的:
- 为了研究和比较不同类型的血液采样中的氨基酸和甲酸水平.
- 以毛细血管全血样本作为参考标准对发现进行评估.
- 评估各种采集管对代谢查分析物的影响.
主要方法:
- 30名成年参与者的血液样本被收集到酸盐,血清分离,肝素和EDTA管以及注射器中.
- 每种采集方法的50μL血液被吸收到过纸上进行DBS分析.
- 指尖刺穿的毛细血管全血也被吸收到过纸上.
- 所有样品都使用流量注射分析双重质谱法 (FIA-MS/MS) 进行分析.
主要成果:
- 几乎所有测量的氨基酸和甲酸水平在指尖DBS组和来自其他采集管的样本之间存在显著差异 (p <0.05).
- 谷氨酸和氨酸是例外,在氨基酸水平上没有显著差异.
- 乙卡尼丁水平的差异不如氨基酸所观察到的差异明显.
结论:
- 使用替代血液采样类型而不是毛细血管全血进行代谢查,可能会导致不准确,特别是较低的结果,特别是对于氨基酸.
- 代谢查的实验室截止值必须考虑所使用的特定血液采样类型.
- 关于采用的采样方法的明确沟通对于准确解释DBS测试结果至关重要.
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