基本螺旋环-螺旋环转录因子25的天然基变异调节了甜中的胡卜素生物合成
Zihao Wei1, Meiqi Shang1, Zhicheng Jiang1
1Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization and Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing, China.
一个关键的基因,IbbHLH25,调节色肉质甜中的胡卜素生产. 其促进物的遗传变异会影响胡卜素水平,为作物生物强化提供潜力.
科学领域:
- 植物分子生物学 植物分子生物学
- 农业科学 农业科学
- 生物化学 生物化学
背景情况:
- 色肉 (OFSP) 对于发展中国家的营养至关重要.
- 了解OFSP中的胡卜素生物合成调节对于提高其营养价值至关重要.
研究的目的:
- 为了确定控制甜中胡卜素生物合成的遗传因素.
- 阐明IbbHLH25基因在胡卜素积累中的作用.
主要方法:
- 转录组和甜加入不同肉色的哈普类型分析.
- 对IbbHLH25和IbbHLH66.6的基因表达分析 (过度表达和沉默).
- 胡卜素含量和组成的分析.
主要成果:
- 在IbbHLH25促销器区域的删除与白色/黄色肉质甜中的胡卜素含量降低有关.
- 过度表达IbbHLH25和IbbHLH66显著增加了总胡卜素,特别是β-胡卜素和β-.
- IbbHLH25和IbbHLH66形成异构体,激活关键的胡卜素生物合成基因.
结论:
- IbbHLH25和IbbHLH66是甜中胡卜素生物合成的关键调节者.
- 这些基因为通过生物强化提高甜的营养质量提供了潜在的目标.
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