评估离子源参数和液态色谱方法用于使用轨道飞行质谱仪的等离子体非目标代谢学
Hailemariam Abrha Assress1, Ahsan Hameed1, Lindsay M Pack2
1Arkansas Children's Nutrition Center, Little Rock, AR, USA; Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
概括
优化质谱仪 (MS) 离子源参数和色谱方法显著增强了非目标代谢. 微调这些条件可以提高信号可重现性,并将血样本中的代谢物识别率提高60%.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 代谢学 代谢学 代谢学
背景情况:
- 非定位代谢学对于理解人类健康和疾病至关重要.
- 强有力的方法开发对于最大限度地减少分析偏差和确保全面的结果至关重要.
- 对于高通量代谢学,需要优化质谱仪 (MS) 参数和染色学.
研究的目的:
- 调查MS离子源参数对信号可重现性和代谢物注释在非目标代谢学中的影响.
- 为了评估不同的移动相梯度和染色学列 (逆相C18和水友相互作用液体染色学) 用于血分析.
- 为了确定最大限度地提高代谢组覆盖范围的最佳条件.
主要方法:
- 系统评估MS离子源参数,包括电喷针定位,喷射电压和气体/温度设置.
- 五个逆相 (RP) -C18和两个水友相互作用液体染色学 (HILIC) 列的比较.
- 使用优化的非目标代谢学工作流程分析血提取物.
主要成果:
- 最佳的电子喷射针位置 (最远的Z方向,最接近的Y方向) 提高了信号可重现性和代谢物注释.
- 为了提高性能,确定了特定的离子源条件 (电压,温度,气体流).
- RP列表现出可比的性能,而一个zwitterionicHILIC列表现出一个胺基HILIC列表现出.
- 结合最佳RP和HILIC方法,与RP单独相比,代谢组覆盖率增加了60%.
结论:
- 微调MS离子源参数对于强大的非目标代谢学至关重要.
- 优化的染色学条件,特别是RP和HILIC的组合,显著扩大了代谢组覆盖范围.
- 这项研究提供了一个框架,用于增强血样本的非向代谢学,以获得更广泛的生物学见解.
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