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糖尿病伤口愈合的突破:theaflavin-3,3'-digallate nanoparticles@hydrogel激活了TGF-β1 / SMAD3通路的作用

Xu Dong¹, Jianyin Miao², Ling Wu¹

¹National Key Laboratory for Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei 230036, PR China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|April 10, 2025

概括

叶黄素-3,3'-二甲酸纳米颗粒 (TFDG NPS) 和TFDG NPS@水凝有效促进糖尿病伤口愈合. 这些纳米颗粒激活TGF-β1 / SMAD3通路,增强原蛋白的产生和加快糖尿病小鼠的伤口关闭.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
伤口治愈研究研究伤口治愈研究

背景情况:

糖尿病严重影响伤口愈合,增加感染风险.
目前糖尿病伤口的治疗选择有限,需要新的治疗方法.

研究的目的:

开发和评估theaflavin-3,3'-digallate纳米颗粒 (TFDG NPS) 和TFDG NPS@hydrogels用于糖尿病伤口治疗的疗效.
阐明TFDG NPS和TFDG NPS@hydrogels促进糖尿病伤口愈合的潜在机制.

主要方法:

TFDG NPS 通过离子交联合成并进行了表征.
在体外生物相容性使用CCK-8测定和活/死染色对HK-2细胞进行了评估.
患有全厚背部伤口的糖尿病小鼠 (STZ诱导) 用TFDG NPS和TFDG NPS@hydrogels进行治疗.
组织学分析,西部斑块和分子对接被用来评估治疗效果和机制.

主要成果:

最佳的TFDG NPS配方是通过TFDG:凝:花素比例为2:1:1实现的.
TEM证实了TFDG球形NPS的直径为~140nm;体外研究显示了有利的生物相容性.
TFDG NPS和TFDG NPS@水凝增强了原沉积,增加了血管化,减少了炎症 (F4/80),并上调了TGF-β1,SMAD3,原I和α-SMA.
分子对接表明TFDG和TGF-β1/SMAD3之间有很高的亲和力.

结论:

在糖尿病小鼠模型中,TFDG NPS和TFDG NPS@水凝显著促进了伤口的关闭.
治疗效果是由TGF-β1 / SMAD3通路的激活介导的,导致增强的原蛋白合成和组织再生.
这些发现凸显了TFDG NPS和TFDG NPS@hydrogels在治疗糖尿病伤口方面的有希望的治疗潜力.

关键词:

原 I 和 α-SMA 的结合物.TFG-β1 / SMAD3 提供服务.糖尿病的伤口正在愈合.亚弗拉-3,3-迪加拉酸纳米颗粒.

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