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将Enterococcus faecium封装在氨酸/凝/酸/质氨酸中可以改善细胞活力和稳定性

Leran Wang¹, Yuqing Sun¹, Shiqi Li¹

¹College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling, Shaanxi 712100, China.

International journal of biological macromolecules

|April 10, 2025

概括

这项研究开发了一种新的方法来保护益生菌,如Enterococcus faecium 20,420使用复合微囊. 封装的益生菌在食品加工和储存过程中显示出增强的生存率,改善了它们的功能性食品潜力.

科学领域:

食品科学与技术食品科学与技术
微生物学微生物学
生物材料工程生物材料工程

背景情况:

由于恶劣的条件,益生菌的生存在食品加工过程中是一个挑战.
菌 (Enterococcus faecium 20,420 (Ef-20,420) 是一种具有显著健康益处的益生菌.
有效的封装对于保持益生菌生存能力至关重要.

研究的目的:

通过物理挤出和复杂的凝聚,提高Ef-20,420的可行性和稳定性.
开发一种复合微囊系统,使用氨酸 (HA),凝 (GL),酸盐 (SA) 和胺硫酸盐 (PS).
评估微囊对加工压力和储存的保护作用.

主要方法:

使用HA,GL,SA和PS复合物的Ef-20,420通过物理挤出和复杂的凝结进行封装.
使用日志CFU/mL对封装前后细胞活性的评估.
微囊形成的特征 (键,静电相互作用).
在模拟胃液 (SGF) 和模拟肠液 (SIF) 下评估益生菌生存率.
在4°C时测试热稳定性和储存稳定性.
对Caco-2细胞跨膜抗性的影响评估.

主要成果:

封装保持了高的Ef-20,420活力 (8.36 ± 0.07日志CFU/mL).
微囊显示出对SGF的显著保护 (1.37 ± 0.13日志CFU/mL减少).
蛋白胺硫酸盐 (PS) 在SIF中延长了60分钟的细菌释放.
高温处理 (80°C3分钟) 导致可活性计数为6.58±0.19日志CFU/mL.
在4°C保存25天后,可生存数量保持在7.10 ± 0.12 log CFU/mL.
复合微囊增强了Caco-2细胞的跨膜抵抗力.

结论:

开发的复合微囊在加工和储存过程中有效地保护Enterococcus faecium 20,420.
封装系统提供了增强的益生菌稳定性和受控释放特性.
这项技术为开发先进的功能性益生菌食品提供了有前途的方法.

关键词:

细胞活力细胞活力复杂的联合化.微囊中的微囊物理挤出物理挤出益生菌益生菌是一种益生菌.

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