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相关概念视频

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.4K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Mutations01:39

Mutations

77.4K
Overview
77.4K
RNA Structure01:23

RNA Structure

70.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
70.6K

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相关实验视频

Updated: May 15, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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SMDesigner:一个设计序列突变以评估RNA结构的程序.

Lijuan Hou1, Meryem Raies1, Kadidia Dite Selly N'Diaye1

  • 1INRS - Institut Armand Frappier.

RNA (New York, N.Y.)
|April 10, 2025
PubMed
概括

我们开发了结构突变设计师 (SMDesigner) 来自动创建用于功能分析的RNA突变. 该工具有助于有效地探索非编码RNA (ncRNA) 的结构功能关系.

科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 遗传学 遗传学 是一个

背景情况:

  • RNA结构对其生物功能至关重要.
  • 深度测序的进步已经确定了许多非编码RNA (ncRNA) 序列,但功能性特征仍然是一个瓶.
  • 传统的研究RNA结构-功能关系的方法涉及艰苦的单一突变分析.

研究的目的:

  • 开发一个自动化程序,结构突变设计师 (SMDesigner),以基于RNA结构信息生成破坏性和补偿性突变.
  • 为了验证SMDesigner在大规模探索RNA结构-功能关系方面的有效性.

主要方法:

  • 开发了结构突变设计师 (SMDesigner) 软件.
  • 应用SMDesigner来设计riboswitch序列的突变.
  • 设计突变的实验验证使用在线探测试验来评估RNA结构和功能.

主要成果:

  • SMDesigner成功生成了预测的RNA突变.
  • 在线探测测试证实了六个测试中的五个核糖开关突变体的结构变化.
  • 这些结果验证了SMDesigner在研究结构RNA功能的实用性.
关键词:
保存结构结构的结构.共同变化 共同变化突变设计的突变设计的结构 ncRNA 结构.寡核酸图书馆 寡核酸图书馆

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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相关实验视频

Last Updated: May 15, 2025

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
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Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

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结论:

  • SMDesigner 是一种用于自动设计RNA突变的有效工具,用于研究结构-功能关系.
  • 该软件有助于对ncRNAs进行大规模的功能分析.
  • 通过简化突变设计和验证,SMDesigner可以加速RNA功能的发现.