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古代蓝藻细菌原生日间钟系统KaiABCBC的重建

Silin Li¹, Zengxuan Zhou¹, Yufeng Wan^1,2

¹School of Life Sciences, Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-sen University, Guangzhou, China.

The EMBO journal

|April 10, 2025

概括

古代的蓝藻细菌适应了地球早期的18小时日,使用原生态循环 KaiABC 系统. 这种古老的系统虽然不自振,但在数十亿年前帮助生物适应更短的光暗周期.

科学领域:

*天体生物学和古生物学
* 分子生物学和生物化学
* 进化生物学进化生物学

背景情况:

*随着时间的推移,地球的旋转速度减慢,导致日夜周期比早期历史更长.
* 古代生物对更短的昼夜周期的适应机制仍然不太清楚.
* 蓝藻细菌拥有昼夜时钟系统 (KaiABC),可以调节每天的生物节奏.

研究的目的:

*重建和分析古老的KaiABC系统 (anKaiABC) 从约950亿年前的蓝藻细菌.
* 了解这种古老的昼夜时钟系统是如何运作的,并促进适应18小时的光/黑暗周期.
* 为了比较古代anKaiABC系统与其现代对应系统的特性.

主要方法:

* 古代KaiABC蛋白质结构和相互作用的生物信息重建.
*生物化学测试以确定KaiC蛋白的酶活性 (激酶,酸酶,ATP酶).
* 功能分析 anKaiABC 系统能够进入明暗周期的能力.
* 用Synechococcus菌株进行基因工程,以表达古代KaiABC基因.

主要成果:

*与现代蛋白质相比,古代AnKaiABC蛋白质的结构和相互作用不同.
* 古代anKaiC显示了减少的激酶,酸酶和ATPase活动.
* 古代的anKaiA和anKaiB蛋白在调节anKaiC酸化方面效率较低.
* anKaiABC系统没有内源性振荡,但可以被18小时的光/黑暗周期所带走.
* 一种表达KaiABC基因的Synechococcus菌株表现出更好地适应9小时的光/9小时的黑暗周期 (LD9:9).

结论:

* 古代anKaiABC系统尽管缺乏自持振荡,但仍然是功能性的,并且可以受到环境线索的影响.
* 这种原生态系统可能在古老的蓝藻细菌适应早期地球18小时昼夜周期的过程中发挥了关键作用.
*这项研究提供了关于昼夜钟的演变和早期生活适应策略的见解.

关键词:

适应适应适应循环节时钟的时间表.蓝藻细菌是一种蓝藻细菌.进化进化进化进化进化进化进化卡伊ABCBC 的意思是

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