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转录基因分析确定CXCL12是子子大小的新型候选基因

Zhiyuan Bao¹, Jie Yang¹, Jiali Li¹

¹College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, People's Republic of China.

Animal bioscience

|April 11, 2025

概括

化学因子连接体 C-X-C 基因化学因子连接体 12 (CXCL12) 促进颗粒细胞的增殖,增加子子的大小. 这种基因是选择在繁殖计划中具有更高生殖潜力的子的一个有希望的标记物.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
动物繁殖动物繁殖

背景情况:

卵巢功能对于调节子的 litter 尺寸至关重要.
卵巢中的基因表达模式影响生殖能力.

研究的目的:

在新西兰子中确定与 litter 尺寸相关的候选基因.
研究基因基因接体C-X-C基因基因接体12 (CXCL12) 在调节颗粒细胞功能和 litter 尺寸中的作用.

主要方法:

卵巢转录基因组测序以比较高和低 litter 尺寸组.
细胞计数套件-8 (CCK-8) 测定和流动细胞计量用于细胞增殖和细胞亡分析.
定量逆转录PCR (qRT-PCR) 和西式涂抹,以评估基因和蛋白质的表达.

主要成果:

CXCL12促进颗粒细胞的增殖,并抑制细胞亡.
CXCL12调节参与细胞循环,增殖和毛囊发育的基因和蛋白质.
通过CXCR4受体,CXCL12激活了JAK/STAT信号通路.

结论:

CXCL12是一种关键基因,通过促进颗粒状细胞的增殖,影响子子的大小.
CXCL12是一种潜在的遗传标记物,用于在繁殖计划中选择子,以改善 litter 尺寸.

关键词:

在CXCL12中,CXCL12是最重要的.在JAK/STAT路径中.垃圾大小垃圾大小垃圾大小卵巢卵巢卵巢卵巢在RNA-seqqq.

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