揭示未经表征的NSP和在动脉病毒中分子工具包的变异的结构和功能影响
Siuli Rakesh1, Kshitij Behera1, Arunkumar Krishnan1
1Department of Biological Sciences, Indian Institute of Science Education and Research Berhampur (IISER Berhampur), Berhampur 760010, India.
NAR genomics and bioinformatics
|April 11, 2025
概括
这项研究通过注释新型蛋白质和识别哺乳动物和非哺乳动物病毒中独特的功能领域,提高了对动脉病毒基因组的理解. 这些发现揭示了不同的进化路径和分子工具包在不同的动脉病毒血统.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 结构生物学 结构生物学
背景情况:
- 动脉病毒基因组有不完整的注释,缺乏关于非结构蛋白 (NSP) 和甲基转移酶 (MTase) 域的细节.
- 新测序的非哺乳动物动脉病毒基因组需要注释和与哺乳动物分离物进行比较.
研究的目的:
- 通过比较基因组学和序列结构分析,增强动脉病毒的基因组架构和蛋白质注释.
- 研究哺乳动物和非哺乳动物动脉病毒谱系中蛋白质和功能域的进化分歧.
主要方法:
- 哺乳动物和非哺乳动物动脉病毒基因组的比较基因组学分析.
- 序列结构分析用于预测蛋白质的功能和域.
- 识别和分类未注释的非结构蛋白 (NSP) 和功能域.
主要成果:
- 确定NSP3 C终端域为有翅膀的螺旋旋转螺旋和NSP7作为一个小的β-桶域,可能是病毒RNA相互作用.
- 归类NSP12作为哺乳动物动脉病毒中衍生的N7-MTase类域变异,缺乏催化残留物.
- 在非哺乳动物动脉病毒中观察到2'-O-MTase和外核酶 (ExoN) 域的零星保留,与哺乳动物分离物相反.
结论:
- 该研究为动脉病毒提供了增强的基因组注释,揭示了谱系特定的进化模式.
- 不同的分子工具包,包括MTase和ExoN域的变异,区分哺乳动物和非哺乳动物的动脉病毒.
- 这些发现有助于进一步了解动脉病毒基因组多样性和进化轨迹.
相关概念视频
00:56Viral Structure
61.2K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
61.2K
02:28Leaky Scanning
5.0K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.0K
00:50What are Viruses?
112.3K
Overview
112.3K
00:36Viral Mutations
32.1K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.1K