EMG1甲基转移酶活性影响KSHV uORFs中的核糖体占用率
Elena M Harrington1, James C Murphy1, Katherine L Harper1
1School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
Cell reports
|April 11, 2025
概括
卡波西的肉瘤相关性疹病毒 (KSHV) 通过专门的机制劫持宿主核糖体. 核糖体因子EMG1在KSHV复制过程中对病毒蛋白质合成和传染性病毒产生至关重要.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 病毒依赖宿主细胞进行蛋白质合成.
- 卡波西的肉瘤相关性疹病毒 (KSHV) 独特地改变宿主核糖体,以产生病毒蛋白质.
- KSHV利用专门的核糖体来翻译病毒转录,包括那些具有上游开放阅读框架 (uORF) 的病毒转录.
研究的目的:
- 调查核糖体生物发生因子EMG1在KSHV溶性复制中的作用.
- 阐明EMG1影响病毒蛋白合成和传染性病毒产生机制.
主要方法:
- 在KSHV感染期间分析EMG1与前体-40S核糖体复合物的关联.
- 通过基因操纵消耗EMG1.
- 评估病毒蛋白表达,流体级联进展和传染性病毒产量.
- 评估EMG1在调节KSHV uORF转化中的甲基转移酶活性.
主要成果:
- 在KSHV光学复制过程中,EMG1与前体-40S核糖体复合体的相关性增强.
- 由于EMG1的减少,病毒蛋白的表达和传染性病毒的产生显著减少.
- EMG1的甲基转移酶活性对于调节晚期溶解基因中KSHV uORFs的翻译至关重要.
结论:
- 通过调节宿主核糖体的组成和功能,EMG1在KSHV临床复制中发挥着关键作用.
- 病毒翻译的EMG1调节对有效的病毒蛋白合成和传染性病毒产生至关重要.
- 准EMG1的甲基转移酶活性可能是对抗KSHV感染的治疗策略.
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