从被关押灵长类动物中分离出来的扩展谱β-乳糖酶产生型大肠杆菌:特征和水平基因转移能力分析
Wenhao Zhong1, Yuxin Zhou1, Mengjie Che1
1College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan, Chengdu, China.
PloS one
|April 11, 2025
概括
在被囚禁的灵长类动物中发现了产生扩展谱β-乳酸酶 (ESBLs) 的大肠杆菌菌株. 这些多药耐药细菌具有水平基因转移的基因,对公众健康构成风险.
科学领域:
- 微生物学与传染病的研究
- 兽医公共卫生 兽医公共卫生
- 遗传学和分子生物学
背景情况:
- 全球扩展光谱β-乳糖酶 (ESBLs) 产生大肠杆菌 (ESBL-EC) 的增加,在人类和动物种群中构成了重大健康挑战.
- 在动物学环境中,被囚禁的灵长类动物可以作为抗微生物耐药细菌的储存库,可能促进它们的传播.
研究的目的:
- 在中国从被囚禁的灵长类动物中分离出来的ESBL-EC菌株的特征.
- 在这个群体中调查横向基因转移 (HGT) 能力和与ESBL-EC相关的遗传元素.
主要方法:
- 通过使用双盘扩散试验,对来自受囚禁灵长类动物的444种多耐药 (MDR) 大肠杆菌菌株进行查.
- 通过PCR检测ESBL,移动遗传元素 (MGE) 和病毒性相关基因 (VAG).
- 结合实验用于评估HGT,以及用于确定人口结构的植物遗传学和MLST分析.
主要成果:
- 确定了69个 (15.54%) ESBL-EC菌株,其中blaCTX-M-55和blaCTX-M-15是最常见的ESBL基因.
- 普遍存在的MGEs包括IS26和ISEcp1,通常在组合中发现 (ISEcp1 + IS26). 常见的VAG是fimC和sitA.
- 超过40%的ESBL-EC菌株表现出结合转移能力,其中IncFIB等离子体占主导地位. 特定的ARG和MGE显示出高的转移率.
结论:
- 囚禁灵长类动物中的ESBL-EC菌株具有显著的HGT潜力,由特定的ARG和MGE驱动,其中分类组B1和ST2161占主导地位.
- 这些发现突出了ESBL-EC作为动物园环境中的潜在威胁,对传播给其他动物,人类和环境产生影响.
- 实施严格的控制措施至关重要,以防止ESBL-EC在被囚禁的灵长类动物种群中传播.
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