一项多omics研究揭示了salidroside在降低TGF-β2诱导的ECM表达中的分子特征和治疗点
Rong Zhang1, Ning Li2, Yuanfu Fan3
1The First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China.
Experimental eye research
|April 11, 2025
概括
沙利化物通过恢复受TGF-β2.2破坏的细胞代谢来抵消初级开角青光眼 (POAG) 中的纤维化. 这种生物活性化合物调节关键分子,为新的POAG治疗提供了潜力.
科学领域:
- 眼科医生 眼科 眼科
- 分子生物学分子生物学
- 细胞的新陈代谢
背景情况:
- 初级开角青光眼 (POAG) 导致全球不可逆转的失明,与状眼网 (TM) 纤维化和眼内压力 (IOP) 升高有关.
- 转化生长因子-β2 (TGF-β2) 加剧TM纤维化和ECM积累,增加水性幽默流出阻力.
- 沙利多在缓解眼睛高血压方面表现有前途,但其在POAG中的分子机制需要阐明.
研究的目的:
- 研究沙利德化物在减轻人类TM细胞中TGF-β2诱导的变化的分子机制.
- 探索TGF-β2和沙利德对TM细胞的转录,蛋白质和代谢特征的影响.
- 为潜在的POAG治疗策略确定由salidroside调节的关键分子标和途径.
主要方法:
- 人类TM细胞被TGF-β2治疗,随后用沙利德.
- 采用了包括转录组学,无标签蛋白组学和非向代谢组学在内的多组学分析.
- 生物信息分析,包括ROC和对接,用于评估salidroside的功能作用.
主要成果:
- TGF-β2治疗抑制了HTM细胞代谢,影响了TCA循环等途径.
- 沙利化物治疗通过通过后转录和转化机制调节15个关键生物分子,包括MELTF和SLC25A10,恢复了代谢平衡.
- SLC25A10被确定为一种潜在的点,涉及代谢运输,能源生产和RNA处理.
结论:
- 沙利德化物有效地对抗人类TM细胞中TGF-β2诱导的代谢功能障碍.
- 该研究揭示了salidroside的治疗潜力,通过调节关键的生物分子和与POAG病变相关的途径.
- 这些发现为开发针对细胞代谢和纤维化的新型POAG治疗提供了基础.
更多相关视频
11:38Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
Published on: September 14, 2021
3.3K
07:49Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
11.2K
相关概念视频
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Mesenchymal Stem Cells
4.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.6K
Cell-surface Signaling
51.3K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
51.3K
The JAK-STAT Signaling Pathway
8.4K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.4K
