相关实验视频

Updated: May 14, 2025

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

在ABO-A2不相容的移植中进行多重珠子免疫试验

Anne M Halpin¹, Cathi Murphey², Bruce Motyka³

¹Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

|April 11, 2025

概括

不兼容ABO-A2的脏移植可以扩大ABO-O和ABO-B受体的获取机会. 一项新的测试表明,抗AII抗体水平,而不仅仅是标位,可以提高这些救命程序的资格.

科学领域:

移植免疫学移植免疫学
血型血清学血型血清学

背景情况:

ABO-A2不兼容 (ABO-A2i) 移植改善了ABO-O和ABO-B受体的获得.
目前的资格依赖于抗A血凝定位,这可能不会准确地反映抗体对抗A-II甘氨酸,血管内皮的关键抗原.

研究的目的:

评估抗A标位是否不准确地限制了具有较低抗A-II甘氨酸抗体的候选人ABO-A2i移植资格.
开发和验证一种用于测量ABO抗体特异性的新试验.

主要方法:

开发了一种单抗原珠子免疫试验,以检测ABO抗体特异性,包括抗A和抗B甘氨酸亚型.
使用对照血清验证了测定特异性和可重复性.
从ABO-B和ABO-O候选人的血清中测量了抗A-II甘氨酸抗体水平,此前对ABO-A2i移植资格进行了评估.

主要成果:

新的测定准确地测量了抗A和抗B甘氨酸亚型特异性抗体.
在141名候选人中,75名候选人因抗A定位 (>4) 而没有资格参加.
在不符合条件的候选人中,55% (41/75) 的抗A-II水平与符合条件的候选人相似 (标位≤4),表明标位与A-II特异性抗体不相关.

结论:

抗A血凝定位是ABO-A2i移植资格的不可靠指标.
开发的ABO抗体测定提供了对移植特异性抗AII抗体的更准确的评估.
这种改进的评估可能会增加ABO-B和ABO-O候选人的移植机会.

关键词:

ABO血球血型组是ABO血球血型组.在ABO-histo兼容性方面.不兼容 ABO 的移植.血凝定位的标位是血凝定位.移植脏移植移植脏多重测试多重测试方法移植不平等移植不平等

更多相关视频

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

Mouse Kidney Transplantation: Models of Allograft Rejection

Mouse Kidney Transplantation: Models of Allograft Rejection

Published on: October 11, 2014

相关概念视频

Blood Transfusion and Agglutination

Blood Transfusion and Agglutination

Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...

Bone Marrow Sampling and Transplants

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...