Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Immunoprecipitation01:20

Immunoprecipitation

5.1K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.1K
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

335
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
335

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Bacterial SOS response as a potential driver of antibiotic resistance gene transfer in wastewater.

Environmental research·2026
Same author

Bacterial Contamination of Autologous Cranial Bone Flaps: Microbiological Analysis and In Vitro Antibiotic Decontamination.

World neurosurgery·2026
Same author

Project ODIN: advancing environmental genomic surveillance for public health across sub-Saharan Africa.

The Lancet. Microbe·2026
Same author

High Concordance of Total Antibody and Transduction Inhibition Assays Enables Replacement of Cell-Based Antibody Testing for Preexisting Anti-AAV Immunity in Cynomolgus Monkey.

The AAPS journal·2026
Same author

In Vivo Clearance of Immune Complexes: Insights Into Human Drug/Anti-Drug Antibody Complex Clearance Dynamics.

The AAPS journal·2026
Same author

Implementation of the fully automated diagnostic immunoanalyzer cobas e411 in the bioanalytical laboratory - Lessons learnt.

Bioanalysis·2026

相关实验视频

Updated: May 14, 2025

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.3K

一种消除IgM免疫测试干扰的工具.

Alexander Pöhler1, Uwe Wessels2, Roland F Staack2

  • 1Roche Pharma Research & Early Development (pRED), Pharmaceutical Sciences, Bioanalysis & Biomarkers, Roche Innovation Center Munich, Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany. alexander.poehler@roche.com.

The AAPS journal
|April 11, 2025
PubMed
概括

一个新的蛋白酶选择性地切割IgM,改善免疫测试. 这种方法消除了类风湿因子干扰,并简化了IgM同型定型,在基因治疗研究中揭示了以前低估的免疫反应.

关键词:
反-AAV测定试验的测定.在IgM干扰的过程中,会出现IgM干扰.在 IgM 蛋白质酶中,IgM 蛋白质酶是免疫测试是一种免疫测试.类风湿病因子干扰干扰

更多相关视频

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
05:25

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

1.3K
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

11.9K

相关实验视频

Last Updated: May 14, 2025

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

28.3K
Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
05:25

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

1.3K
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

11.9K

科学领域:

  • 生物化学 生物化学
  • 免疫学 免疫学 免疫学
  • 分析化学 分析化学

背景情况:

  • 免疫测试对于生物分析至关重要.
  • 类风湿因子 (RF) 和治疗出现的IgM可以干扰免疫检测结果.
  • 准确量化免疫反应至关重要,特别是在基因疗法研究中.

研究的目的:

  • 评估一种新的IgM选择性蛋白酶,用于生物分析应用.
  • 证明蛋白酶消除射频干扰的能力,并简化IgM异型鉴定.
  • 调查治疗出现的IgM对抗囊IgG测定的影响.

主要方法:

  • 使用一种新开发的IgM选择性蛋白酶.
  • 在三种不同的生物分析场景中应用蛋白酶处理.
  • 在选择性IgM消化之前和之后分析了免疫测试结果.

主要成果:

  • 蛋白酶治疗成功消除了类风湿性因素干扰.
  • 在蛋白酶应用后,IgM异型鉴定得到了显著的简化.
  • 选择性IgM消化在抗囊IgG试验中解决了被低估的免疫反应,这是由治疗引起的IgM引起的.

结论:

  • 选择性IgM蛋白质分解裂变是提高免疫检测性能的一个有价值的工具.
  • 这种蛋白酶有效地消除了常见的干扰,提高了测定精度,简化了复杂的分析.
  • 这些发现强调了在基因疗法免疫性评估中解决IgM干扰的重要性.