相关实验视频

Updated: Jun 7, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

非常动态和敏感的NEMOer指标用于在易激发细胞中成像ER信号

Wenjia Gu¹, Jia-Hui Chen², Yiyin Zhang³

¹Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.

Nature communications

|April 11, 2025

概括

研究人员开发了NEMOer,敏感的有机细胞遗传编码指标 (GECI),以检测活细胞内 плазма内/血内网膜 (ER/SR) 中的基本释放事件.

科学领域:

细胞生物学细胞生物学
生物化学生化学
身体生理学身体生理学

背景情况:

细胞内膜/细胞内膜网膜 (ER/SR) 对于 (Ca2+) 信号传递至关重要.
现有的基因编码的Ca2+指标 (GECI) 难以检测ER/SR的基本Ca2+释放事件,特别是在肌肉细胞中.

研究的目的:

开发新的细胞器GECI (NEMOer) 用于敏感检测ER/SR Ca2+动态.
为了实时监测Ca2+稳态和基本释放事件.

主要方法:

开发和描述一套名为NEMOer.的新一套有机细胞GECI.
与现有指标 (例如G-CEPIA1er) 相比,测试NEMOer的灵敏度和动态范围.
使用比度NEMOer进行超分辨率监测并检测心肌细胞中的Ca2+闪和斑马鱼中的体内.

主要成果:

与G-CEPIA1er相比,NEMOer的指标显示出一个数量级更大的动态范围.
在非刺激细胞和刺激细胞中,NEMOer实现了2.7倍更敏感的Ca2+过渡体检测.
在斑马鱼中,NEMOer-f成功检测了心肌细胞SR中的初级Ca2+闪和斑马鱼中自发的SR Ca2+释放.

结论:

NEMOer传感器显著提高了捕获ER/SR Ca2+动态的能力,具有高灵敏度和响应能力.
这些新型传感器允许前所未有的实时,时空监测复杂的Ca2+信号传递和活细胞中的恒温.
NEMOer扩展了研究器官Ca2+信号的工具包,特别是基本释放事件.

更多相关视频

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Published on: October 4, 2018

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

相关概念视频

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...