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在人类视网膜有机体中,SARS-CoV-2尖端蛋白暴露对视网膜发育的影响
Jing Gong1,2,3, Lingling Ge1,2, Yuxiao Zeng1,2
1Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
Cell & bioscience
|April 11, 2025
概括
严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 尖端蛋白在早期和晚期不同地影响人类胚胎视网膜器官发育. 这项研究揭示了明显的分子和细胞变化,为潜在的视网膜损伤提供了洞察力.
科学领域:
- 眼科医生 眼科 眼科
- 发展生物学 发展生物学
- 病毒学 病毒学
背景情况:
- 孕妇有高风险感染SARS-CoV-2,这可能会影响胚胎发育.
- SARS-CoV-2 感染与视网膜病理变化和胚胎视网膜感染有关.
- SARS-CoV-2 尖峰 (S) 蛋白调解宿主细胞的进入和致病,但其对视网膜发育的具体影响尚不清楚.
研究的目的:
- 研究SARS-CoV-2 S蛋白暴露对不同阶段人类视网膜发育的影响.
- 阐明S蛋白诱导的视网膜损伤的潜在细胞和分子机制.
主要方法:
- 使用人类胚胎干细胞衍生的视网膜器官 (hERO) 作为模型系统.
- 在早期 (第90天) 和晚期 (第280天) 发育阶段暴露于S蛋白的hERO.
- 采用免疫光染色,RNA测序和RT-PCR来分析影响.
主要成果:
- 对于SARS-CoV-2进入至关重要的ACE2和TMPRSS2受体在hERO中表达.
- 在早期和晚期的hERO中,S蛋白暴露引发了炎症反应.
- 早期的S蛋白暴露改变了核成分和脂质代谢;晚期的暴露影响了细胞膜和细胞外基质.
结论:
- 根据暴露的阶段,SARS-CoV-2 S蛋白对视网膜发育有不同的影响.
- 这些发现提供了对人类视网膜中SARS-CoV-2诱导的发育异常机制的见解.
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