转录组分析显示,在水果成熟过程中,Cerasus humilis的周骨和肉质之间有机酸代谢的明显差异
Bingcheng Guo1, Li Zhang1, Jinli Guo1
1College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010010, China.
Plants (Basel, Switzerland)
|April 12, 2025
概括
果酸和酸等有机酸在Cerasus humilis水果中的含量高于肉质. 酶活动和基因表达解释了这些差异,影响了水果的质量.
科学领域:
- 植物生理学 植物生理学
- 果实生物化学 果实生物化学
- 代谢学 代谢学 代谢学
背景情况:
- 有机酸对于水果的味道,风味,质量和营养价值至关重要.
- 果皮和果肉之间存在有机酸代谢的显著差异.
- 了解这些差异是提高水果质量的关键.
研究的目的:
- 为了研究在成熟过程中果实皮与Cerasus humilis的果肉中的有机酸的代谢区别.
- 确定负责差异性有机酸积累的关键酶和基因.
- 为了关联有机酸含量,酶活性和基因表达.
主要方法:
- 在五个发育阶段对皮和纸中的酸和酸含量进行分析.
- 测量相关的代谢酶活动.
- 转录组分析以评估基因表达趋势.
- 对所有测量指标的相关性分析.
主要成果:
- 酸是占主导地位的有机酸,其骨中的含量明显高于肉质.
- 酸含量在周心中也更高.
- 特定的酶 (PEPC,NAD-MDH,NADP-ME,CS,ACO) 和基因 (ChMDH2,ChME,ChCS2,ChCS3,ChACO1,ChACO2) 被确定为这些差异的关键调节者.
结论:
- 腹地内较高的有机酸含量有助于其总体酸度更高.
- 和肉之间的不同酶活动和基因表达模式调节了Cerasus humilis中的有机酸积累.
- 这些发现提供了对水果质量特征的遗传和代谢基础的见解.
更多相关视频
11:17A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
34.8K
09:38Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
8.5K
相关概念视频
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
