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Updated: Jun 20, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
09:18

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Published on: January 29, 2019

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通过近距离标记来研究神经元子组件的分子组成

Mareike Lohse1,2, Siqi Sun1,3, Maksims Fiosins4

  • 1Synapse Biology Group, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany.

Methods in molecular biology (Clifton, N.J.)
|April 12, 2025
PubMed
概括

这项研究详细介绍了在小鼠神经元中使用BioID的近距离标记协议. 这种方法有助于绘制蛋白质复合体的地图,并了解神经元功能和可塑性.

关键词:
关联性净化净化方法生物化鉴定 (BioID) 是一种方法.质谱测量质量谱测量主要神经元的主要神经元.靠近标签的标签.突触 (Synapses) 是一个突触的组成部分.这是一个TurboIDID.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...