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Updated: May 14, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
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Published on: March 24, 2014

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用STED显微镜成像突触超结构和组织.

Marta Maglione1,2,3, Stephan J Sigrist4,5

  • 1Institute for Biology, Freie Universität Berlin, Berlin, Germany.

Methods in molecular biology (Clifton, N.J.)
|April 12, 2025
PubMed
概括

超分辨率显微镜解决了神经元突触中的纳米级蛋白质组织. 本研究详细介绍了一种使用时隔STED显微镜的方法,以在现场与通道相对地映射活性区蛋白质.

关键词:
活动区 活动区 活动区脑部切片 脑部切片在Cav2.1中使用.莫西纤维是一种纤维.在RIM1中,RIM1是指RIM1.超级分辨率的超级分辨率突触突触是一个突触突触.gSTED 的意思是什么?

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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