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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

用于固定T细胞中的超分辨率显微镜的成像协议.

Miriam Kopdag¹, Lola C Hernandez¹, Anette Rosche¹

¹The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Methods in molecular biology (Clifton, N.J.)

|April 12, 2025

概括

此摘要是机器生成的。

超分辨率显微镜,使用刺激辐射耗尽 (STED),允许详细可视化T细胞结构. 本协议详细介绍了成像和处理,以提高对T细胞生物学的理解.

关键词:

是一种在IP3R中,IP3R是IP3R.免疫光效应免疫光效应这里是Orai1 .子一个子在T细胞,T细胞.

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Published on: March 22, 2012

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Published on: March 22, 2012

科学领域:

细胞生物学细胞生物学
显微镜的使用方法
免疫学免疫学免疫学

背景情况:

传统的光显微镜被衍射限制,阻碍了纳米尺度可视化.
超分辨率显微镜克服了细胞成像的这些限制.
了解T细胞结构对于免疫学至关重要.

研究的目的:

提供固定初级小鼠T细胞超分辨率成像的详细方案.
为了使T细胞基结构的纳米尺度可视化.
促进对T细胞生物学和组织的更深入的了解.

主要方法:

准备和染色固定原始小鼠T细胞的协议.
超高分辨率成像使用刺激发射耗尽 (STED) 显微镜与共聚焦激光扫描.
使用解卷和细分的图像数据的后处理.

主要成果:

重建了T细胞结构的高分辨率图像.
对T细胞内部组织和相互作用的详细分析成为可能.
该协议能够在纳米范围进行可视化.

结论:

超高分辨率的STED显微镜提供了前所未有的T细胞结构细节.
该协议通过揭示复杂的细胞组织,增强了对T细胞生物学的研究.
该方法有助于更深入地了解T细胞的功能和相互作用.