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Updated: May 14, 2025

Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Published on: June 19, 2010

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通过纳米电子喷雾电离和质子转移减轻电荷使得充满和空的AAV5粒子的质量确定成为可能

Nicolas J Pizzala1, Boukar K S Faye1, Hangtian Song2

  • 1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.

Analytical chemistry
|April 12, 2025
PubMed
概括

准确确定病毒等大型生物分子的质量是具有挑战性的. 这项研究使用纳米电子喷雾电离和离子/离子反应来解决电荷状态,从而能够精确地测量高达4.5兆达尔的腺相关病毒颗粒的质量.

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Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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