在生物样本中开发对有害轮胎抗氧化剂6PPD的定量分析方法,用于毒性评估
Min-Seok Choi1, Sung-Hwan Kim2, Moonjung Hyun3
1Department of Environment & Energy, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk State 54896, Republic of Korea.
Ecotoxicology and environmental safety
|April 12, 2025
概括
一种新的分析方法可以在生物样本中准确量化N-1,3-二甲基丁) -N'--p-二胺 (6PPD). 这有助于理解6PPD暴露和管理其环境和健康风险.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- N-(1,3-二甲基) -N étaire-phenyl-p-phenylenediamine (6PPD) 是一种轮胎抗氧化剂,具有显著的环境暴露问题.
- 6PPD暴露的有效管理需要在各种矩阵中使用敏感和可靠的量化方法.
- 现有的方法可能缺乏在生物样本中超微量水平检测的灵敏度或适用性.
研究的目的:
- 开发和验证一种高灵敏度的分析方法来量化6PPD.
- 在小鼠肝脏样本中建立6PPD分析的预处理协议.
- 为了研究暴露后在小鼠肝脏中的6PPD残留物分布.
主要方法:
- 具有紫外线检测 (HPLC-UV) 的高性能液体染色学,使用乙和脱离离子水作为移动相.
- 开发一种用于小鼠肝脏样本制备的预处理方案.
- 验证该方法的线性,检测极限 (LOD),量化极限 (MDL),恢复和精度.
主要成果:
- 开发的HPLC-UV方法显示出极好的线性 (R2 = 0.9999) 与超微量检测能力 (LOD:0.17 pg/μL,MDL:5.51 pg/μL).
- 肝脏预治疗方案实现了较高的相对恢复率 (71.89%) 和精度 (RSD = 2.20 ± 0.92%).
- 对小鼠肝脏样本的分析显示,暴露后9小时6PPD残留物显著增加,依赖于剂量 (R2 = 0.9906).
结论:
- 在环境和生物矩阵中成功开发和验证了一种具有成本效益和可靠的分析方法,用于6PPD量化.
- 该方法为评估6PPD暴露动态提供了一个实用的工具.
- 调查结果有助于更好地了解6PPD风险,并支持制定环境和人类健康保护的监管准则.
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