在A53T转基因小鼠中,Withaferin A通过多omics综合分析维持了微生物组和代谢组特征
Xiaodong Sun1, Bingqing Qin2, Ai Guo1
1Research Center for Translational Medicine, Hubei Provincial Clinical Research Center for Parkinson's Disease at Xiangyang No.1 People's Hospital, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan, 442000, PR China.
概括
维他费林A (WFA) 通过增强肠道细菌和调节大脑脂通路来改善帕金森病 (PD) 症状,通过微生物群-肠道-大脑轴提供一种新的治疗方法.
科学领域:
- 神经科学是一个神经科学.
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 帕金森病 (PD) 涉及运动和胃肠道问题.
- 维他费林A (WFA) 显示了PD的治疗潜力.
- 目前尚不清楚WFA对肠道微生物群代谢的影响.
研究的目的:
- 在PD小鼠模型中研究WFA的神经保护机制.
- 检查WFA对肠道微生物群组成和新陈代谢的调节.
- 将WFA的影响与大脑空间代谢学相关联.
主要方法:
- 用过的SNCA转基因 (A53T) 小鼠接受了WFA治疗.
- 通过行为测试,免疫组织化学,西斑和ELISA评估运动/认知功能和PD病理学.
- 分析了肠道微生物群 (16S rRNA),便和大脑空间代谢.
主要成果:
- WFA改善了运动性能,认知,肠道屏障,并减少了神经炎症.
- 在WFA中增加了有益的肠道细菌 (Bifidobacterium,Dubosiella,Akkermansia).
- 在WFA中改变了便代谢物 (脂,血清路) 和脑脂信号传递 (Sphk1,S1PR5).
结论:
- 在PD模型中,WFA表现出神经保护作用.
- WFA调节肠道微生物群和大脑新陈代谢.
- WFA的机制涉及微生物群-肠-大脑轴内的脂体信号通路.
相关概念视频
01:22Introduction to the Human Microbiota
222
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
222
01:27Microbiota of the Large Intestine
108
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
108